Fig. 3
Cell viability changes in cancer cell lines after knockdown of iCell rewired genes. For each of MCF7 breast cancer (a), PC-3 prostate cancer (b), A549 lung cancer (c), and HCT116 colon cancer (d) cell lines, cells were seeded in triplicates into 96-well plates and were transfected with indicated esiRNAs 24 h after seeding. The esiRNAs correspond to the 20 top iCell hits minus HLA-DQA2 (because it was not in our library), to which we added esiLuciferase as control. Seventy-two hours after transfection, Presto Blue cell viability assays were performed. Cell viability is calculated relative to the corresponding esiLuciferase controls (set to 100%). For each cell type and for each gene, bars represent the average of the relative changes of cell viability over the triplicate experiments, and the error-bars show the corresponding standard deviations. Bars in blue indicate reduced cell viability in cancer that is statistically different from the control (MWU p value ≤ 5%), bars in red indicate increased cell viability in cancer that is statistically different from the control, and bars in white indicate cell viability changes in cancer cell lines that were not statistically significant
