Fig. 2
From: Decoding the 5′ nucleotide bias of PIWI-interacting RNAs
The identity of Piwi’s SL is not required for Piwi’s function in vivo. a Flies expressing Gal4 instead of Piwi from the piwi-promoter ([piwi]>Gal4) are heterozygous fertile, but homozygous sterile. A Gal4-inducible Piwi transgene (UASp_FH-Piwi(wt)) and select SL mutants rescue fertility and ovarian morphology. b Localization of FH-Piwi-SL transgenes (IF: anti-HA) and all Piwi (IF: anti-Piwi) in piwi heterozygous (*) ovaries. c Nucleotide (nt) size distribution of piRNAs that associate with FH-Piwi wt (gray) and SL mutants. [PiRNAs associated with FH-Piwi-SL(wt), FH-Piwi-SL(Ago3), and FH-Piwi-SL(GSS) were purified from rescued piwi null ovaries. FH-Piwi-SL(LD)-piRNAs were purified from piwi heterozygous flies (*)]. Scale bars indicate 20 µm. d Annotation of piRNAs by their potential to target different transposon families by antisense complementarity. e Genomic origin of piRNAs with respect to annotation categories in sense (s) and antisense (as) orientation. f Nucleotide frequencies at the first position of FH-Piwi-SL piRNAs. [M ≤ 100: Unique mapping reads and primary alignments of multimapping reads are considered for the analyses represented in d–f; read length was restricted to 24–29 nt for analyses presented in d–f.] (See also Supplementary Fig. 2 and Supplementary Data 4.)
