Fig. 3
From: Orthobunyavirus spike architecture and recognition by neutralizing antibodies
The SBV Gc head domain is the major target of neutralizing antibodies in its natural hosts. a Antibody subpopulations were depleted from three cattle sera (left) and one sheep serum (right) collected 28 days after experimental infection with SBV. The above-indicated recombinant depletion antigens were head–stalk (aa 465–874), head (aa 465–702), stalk (aa 694–874), and core (aa 890–1326). The histogram shows ELISA reactivity after depletion with the full Gc ectodomain (aa 465–1326, top) or with the respective depletion antigens (below). Bars represent mean percentages of mock-depleted sera, the optical density values of which ranged robustly between 3.2 and 3.6 for reactivity with the Gc ectodomain. Owing to saturation effects, ELISA reactivity is likely not linearly proportional to the absolute binding activity in a sample. b Virus neutralization after depletion with the indicated antigens for the three cattle sera (left) and the sheep serum (right). Bars represent mean percentages of mock-depleted sera. Dots represent technical replicates. Statistical analysis of the neutralization data was performed using the Mann–Whitney rank sum test. **P < 0.01; ***P < 0.001; n.s. not significant (P > 0.05). Source data are provided as a Source Data file
