Fig. 4
From: Orthobunyavirus spike architecture and recognition by neutralizing antibodies
Immunization with the SBV head–stalk fragment protects IFNAR−/− mice from viremia. a, b Survival curves (a) and relative body weight development (b) of vaccinated and unvaccinated mice over the first 9 days post infection (n = 6 per group). The immunization antigens were head–stalk (aa 465–874) and head (aa 465–702). Weights are given as mean percentage ± s.d. c Viral genome loads at 3, 7, and 21 days post infection (dpi) or on the day when an unvaccinated animal had to be euthanized (fatalities). Genome equivalent concentrations were determined in EDTA–blood samples by S segment-based RT-qPCR. Horizontal bars represent mean values. The dashed line indicates the detection limit (5 × 102 copies/mL). Statistical analysis was performed using the Mann–Whitney rank sum test. *P < 0.05; **P < 0.01; n.s. not significant (P > 0.05). d Percentage of animals positive for N protein-specific antibodies at 21 dpi or on the day when an unvaccinated animal had to be euthanized, as determined by ELISA. The numbers of seroconverted animals in each category is indicated. #One unvaccinated animal naturally succumbed to the infection and was thus not available for blood analysis. Source data are provided as a Source Data file
