Fig. 5
From: Orthobunyavirus spike architecture and recognition by neutralizing antibodies
The two previously identified antigenic sites on SBV Gc map to the trimer interface. a Steady-state binding curves for the recombinant Gc head–stalk construct binding to immobilized mAbs 1C11 and 4B6 in biolayer interferometry. Data were acquired in duplicates and are displayed as mean ± s.d. Source data are provided as a Source Data file. b Focus reduction neutralization (FRNT) of ~750 focus-forming units per mL SBV isolate BH80/11-4 with mAbs 1C11 and 4B6 on Vero cells. Data were acquired in duplicates and are displayed as means ± s.d. Negative neutralization values indicate a slight enhancement of infection with respect to a control without antibodies. Source data are provided as a Source Data file. c Model of the single-chain variable fragments (scFvs) of 1C11 (magenta) and of 4B6 (orange) bound to a single protomer of the SBV spike that is docked into the electron cryo-tomography map of the trimeric BUNV glycoprotein spike17. The illustration was compiled from two separate crystal structures of the head domain in complex with each scFv. d Detail of the interaction interface with 1C11. e Detail of the interaction interface with 4B6. Residues on both sides of the interface are labeled and are shown as sticks with oxygen atoms in red and nitrogen atoms in blue. Secondary-structure elements and disulfide bonds are labeled as in Fig. 1
