Fig. 6
From: Impact of pre-existing dengue immunity on human antibody and memory B cell responses to Zika
Depletion of DENV antibodies in serum does not have a significant effect on ZIKV neutralization. Neutralization analysis of DENV-depleted serum samples from ZIKV-infected patients with no (DENV-naïve/ZIKV), one (DENV-ZIKV), or more than one (+DENV-ZIKV) prior DENV infection. a ZIKV neutralizing antibody titer (FRNT50) in DENV-depleted versus control (BSA)-depleted samples for each group of ZIKV-infected patients at ~8 months postinfection. Lines connect results from each individual. b Statistical analysis of ZIKV neutralizing titers before and after depletion of DENV-reactive antibodies at ~8 months postinfection. Statistical analysis was performed by Wilcoxon test, but no significance differences were found among nondepleted and DENV-depleted ZIKV neutralizing antibody titers. c ZIKV neutralization antibody titers before and after depletion of DENV-reactive antibodies from three representative subjects analyzed in a. d Analysis of ZIKV type-specificity (TS) and ZIKV and DENV cross-reactivity (CR) in total ZIKV neutralizing response. Median and IQR is shown for d, and significance was determined by Mann–Whitney test. *p < 0.05, **p < 0.01, ***p < 0.001. Neutralizing antibody titers were log10 transformed for the analysis of a–c. DENV-naïve/ZIKV, n = 10; DENV-ZIKV, n = 10; +DENV-ZIKV, n = 8
