Fig. 6
Nup2 and Sir2 also regulate regional Hop1 distribution in prophase. a Hop1 ChIP-seq enrichment in late prophase cells (T = 6 h) from nup2Δ ndt80Δ in magenta is plotted along each of the 16 yeast chromosomes; black triangles mark the centromeres and the red hexagon marks the rDNA locus. The data are normalized to a global mean of 1. Inset shows mean Hop1 enrichment in EARs (20–110 kb) in nup2Δ ndt80Δ during early prophase (T = 3 h, pink) and late prophase (T = 6 h, magenta) compared to ndt80Δ samples. ***P < 0.001, and n.s. not significant, Mann-Whitney-Wilcoxon test. b Mean Hop1 enrichment in the EARs (32 domains) is plotted as a function of the distance from telomeres in nup2Δ ndt80Δ. c Bootstrap-derived distributions within two 90-kb bins per chromosome from Hop1 ChIP-seq data illustrated as violin plots for nup2Δ ndt80Δ (pink), and sir2Δ ndt80Δ (blue). The horizontal lines in the violin plots represent the median and the two-ended 95% CIs. The mean Hop1 ChIP-seq enrichment in EARs (20–110 kb) for the respective samples is shown as orange/red dots. The gray dashed line is genome average. d Bootstrap-derived distributions within single 2-kb bins per chromosome are illustrated as violin plots for Hop1 ChIP-seq data from nup2Δ ndt80Δ (pink), and sir2Δ ndt80Δ mutants (blue) are illustrated as violin plots. The horizontal lines in the violin plots represent the median and the two-ended 95% CIs. The mean Hop1 ChIP-seq enrichment around centromeres (2 kb) for the respective samples is shown as orange/red dots. e Hop1 ChIP-seq enrichment in early prophase cells (T = 3 h) from sir2Δ ndt80Δ in light blue is plotted along each of the 16 yeast chromosomes; black triangles mark the centromeres and the red hexagon marks the rDNA locus. The data are normalized to a global mean of 1. Inset shows mean Hop1 enrichment in EARs (20–110 kb) in sir2Δ ndt80Δ during early prophase (T = 3 h, light blue) and late prophase (T = 6 h, dark blue) compared to ndt80Δ samples. **P < 0.01, and n.s. not significant, Mann-Whitney-Wilcoxon test. f Mean Hop1 enrichment in the EARs (32 domains) is plotted as a function of the distance from telomeres in sir2Δ ndt80Δ
