Fig. 8
Enrichment of Hop1 and DSB markers on short chromosomes increases significantly in late prophase. a Schematic of telomere-adjacent enrichment of Hop1 in late prophase predicts bias for DSBs on short chromosomes. The orange bars illustrate the large domains (~100 kb) of long-lived DSB hotspots at telomere-adjacent and rDNA-adjacent regions. Interstitial regions (magenta) harbor mainly the short-lived DSB hotspots. b–g Mean ChIP-seq enrichment per kb is plotted for each chromosome on log scale with regression analysis. P and R2 values are noted below the sample name. R2, measure of the fit of the points to the line, can vary from 0 to 1.0 with 1.0 indicating a perfect fit. P is the probability of obtaining large R2 values. Two-way ANOVA was performed to test significant difference in the slope between the regression lines for different ChIP-seq samples. ANOVA-derived P values are indicated, ***P < 0.001. γ-H2A ChIP-seq enrichment in early (T = 3 h) and late prophase (T = 6 h) in ndt80Δ and late prophase (T = 6 h) in spo11Δ ndt80Δ in b. Hop1 ChIP-seq in early (T = 3 h) and late prophase (T = 6 h) in ndt80Δ samples (c). Hop1 ChIP-seq in late prophase from ndt80Δ samples (T = 6 h) is plotted in orange (d). Plot in magenta is Hop1 enrichment from telomere-distal regions lacking EARs (110 kb from each chromosome end). Error bars are standard deviation of the means of 10 equal sized bins for each chromosome. Late prophase (T = 6 h) enrichment of Hop1 in ndt80Δ and pch2Δ ndt80Δ cultures (e). Hop1 ChIP-seq in early (3 h) and late prophase (T = 6 h) in nup2Δ ndt80Δ samples (f). Hop1 ChIP-seq in early (T = 3 h) and late prophase (T = 6 h) in sir2Δ ndt80Δ samples (g)
