Fig. 2 | Nature Communications

Fig. 2

From: G-quadruplex DNA drives genomic instability and represents a targetable molecular abnormality in ATRX-deficient malignant glioma

Fig. 2

ATRX deficiency promotes replication stress and DNA damage. a γ-H2AX immunofluorescence in constitutive (upper panel) and inducible (lower panel) NHAs (DAPI counterstain). b Quantified percentage of cells with >10 immunopositive γ-H2AX foci (50 nuclei counted in all cases). c NHAs with ATRX knockdown were double stained for G4 and 53BP1, showing extensive colocalization. d Western blots of p-KAP1 and p-CHK1 show activation of replication stress arising with ATRX knockdown. e γ-H2AX and BLM ChIP in shCon and shATRX1 NHAs (three replicates each) showing that ATRX deficiency promotes enrichment for both proteins at putative G4-rich promoter regions (MYC and ZNF618) far exceeding that seen at a negative control site (ESR1). Where applicable, error bars reflect SEM; P values determined by unpaired, two-tailed t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s.: P > 0.05); scale bars represent 10 µm

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