Fig. 6 | Nature Communications

Fig. 6

From: G-quadruplex DNA drives genomic instability and represents a targetable molecular abnormality in ATRX-deficient malignant glioma

Fig. 6

G4 stabilization selectively promotes apoptosis and synergizes with DNA-damaging therapies. a, b Time course study in shCon and shATRX1 NHAs (three replicates each) treated with 100 nM CX-3543 showing parallel kinetics of apoptosis (Annexin V positivity) (a) and p-CHK1/p-KAP1 levels by western blot (b). c, d Analogous time course study in inducible shATRX2 cells (three replicates each) documenting p-CHK1 an p-KAP1 levels (d) by western blot and Annexin V positivity (c) over time. e, f Soft agar colony counts for NHAs (3 replicates each) treated with either vehicle control (Veh) or 50 nM CX-3543 and either increasing doses of IR (e) or HU (f). Colony counts measured at 21 days scaled to that of shCon, Veh NHAs. Where applicable, error bars reflect SEM; P values determined by two-way ANOVA (*P < 0.05, **P < 0.01)

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