Fig. 7
From: Rapid active zone remodeling consolidates presynaptic potentiation
Mutants incapable of PHP impair short-term memory. a Confocal images of adult Drosophila mushroom-body regions of control (top; undriven UAS-Unc13ARNAi (UAS-Unc13ARNAi::+)) and rescue of driven UAS-Unc13ARNAi with C-term-GFP (bottom; Ok107::UAS-C-term-GFP::UAS-Unc13ARNAi) brains labelled with the indicated antibodies. b Averaged odor responses measured at the level of presynaptic boutons of M4/6 (MBON-β‘2 mp/MBON-γ5β′2a/MBON-β2β′2a) mushroom body output neurons (compare ref. 54 or ref. 89). Above: sample images of two-photon recordings from M4/6 cells (20 frames averaged respectively) before and after odor onset. Grey shading indicates the time at which the odor was applied. Left panels OCT and right MCH response. Below: averaged odor responses. Five responses per odor were averaged per animal. Solid lines show mean responses (n = 5–6 animals per genotype). Shaded areas represent the SEM. c Short-term memory scores after mushroom body-specific C-term-GFP rescue after Unc13A downregulation via locally driven RNAi expression (Ok107::UAS-C-term-GFP::Unc13ARNAi) compared to controls expressing the driver, but not the RNAi (Ok107::+), the RNAi without driver (UAS-Unc13ARNAi::+) or mushroom body-specific overexpression of the C-term-GFP construct (Ok107::UAS-C-term-GFP). See also Supplementary Fig. 9. Source data as exact raw values, detailed statistics including sample sizes and P values are provided in the Source Data file. Scale bars: a 20 µm; b 10 µm. Statistics: nonparametric one-way analysis of variance (ANOVA) test, followed by a Tukey’s multiple comparison test. ***P ≤ 0.001; n.s., not significant, P > 0.05. All panels show mean ± s.e.m. For representative images experiments were repeated twice with at least 6–7 brains per genotype
