Fig. 4

Loss of Regnase1 causes impaired hematopoietic functions. a Kaplan-Meier survival curve of control (fl/fl) and Regnase-1-KO (Δ/Δ) mice (n = 11). P values were calculated by the logrank test. b Representative gross appearance of the spleen and mesenteric lymph nodes (MLNs) collected from 8-week-old mice as indicated. The scale bars represent 1 cm. c Spleen (right) and MLNs (left) weight of 8-week-old mice as indicated (n = 4 per group). d Flow cytometric analysis of lymphocytes and monocytes in PBMCs from 8-week-old mice as indicated (n = 3 per group; 3 independent experiments). e Quantification of frequencies of lymphoid and myeloid cells in PBMCs. f Flow cytometric analysis of lymphocytes and monocytes in BM MNCs from 8-week-old mice as indicated (n = 3 per group; 3 independent experiments). g Quantification of frequencies of lymphoid and myeloid cells in bone marrow MNCs. h Kaplan-Meier survival curve of control (fl/fl) and Regnase-1 hetero-mutant (fl/Δ) mice (n = 11). P values were calculated by the logrank test. i Representative images of the spleen and MLNs collected from 12-month-old mice as indicated. The scale bars represent 1 cm. j Spleen (right) and MLNs (left) weight of 12-month-old mice as indicated (n = 4 per group). k, l Representative flow cytometric analysis and quantitative evaluation of LSK-HSC numbers in the BM of 8-week-old mice as indicated (n = 3 per group; 3 independent experiments). m Quantification of ratios of lymphocytes and monocytes in PB (left) and BM (right) MNCs from 12-month-old mice as indicated (n = 3 per group; 3 independent experiments). The ratio was calculated by flow cytometric analysis. Error bars indicate mean ± SD. *p < 0.05; **p < 0.01, ***p < 0.005, logrank test in h, two-sided t-test in c, e, g, j, m, l