Fig. 3
LasB induces a pro-inflammatory response in the absence of microbiota. a Protocol for monocolonization of C57BL/6 mice with P. aeruginosa PA14 wild-type (WT) or the lasB mutant. b P. aeruginosa bacterial load recovered in the small intestine of WT (n = 6) and lasB mutant (n = 5) colonized mice 3 weeks post colonization. c Luminal small intestinal glutenasic activity from mice monocolonized with P. aeruginosa PA14 WT (n = 6) or the lasB mutant (n = 5). Representative bioassays are shown. d Luminal small intestinal elastase activity from mice monocolonized with P. aeruginosa PA14 WT (n = 6) or the lasB mutant (n = 5). e Quantitative measurement of intraepithelial lymphocytes (IELs)/100 enterocytes in small intestinal villi tips of P. aeruginosa PA14 WT (n = 6) or lasB mutant (n = 5) monocolonized mice. f Heat map of gene expression in the small intestinal IEL compartment of mice colonized with P. aeruginosa PA14 WT (n = 3) or the lasB mutant (n = 3) assessed by NanoString nCounter gene expression. Only statistically different genes generated by nSolver 2.5 using Student’s t-test and based on bacterial colonization are shown. Data for b–e are presented as mean ± s.e.m. where each dot represents an individual mouse. Displayed P values were calculated by Student’s t-test
