Fig. 4
Examination of membrane protein lateral mobility on GCs. a Representative trajectories from CD80-GFP fluorescence tracking on different GCs and control cells examined by TIRF microscopy. b Diffusion coefficients of CD80-GFP were calculated from the TIRF fluorescence tracking data. Error bars represent geometric mean with 95% confidence interval. c Representative images showing recovery of CD80-GFP fluorescence in 4 wt% GC following photobleaching. Red rectangles indicate the photobleached area of interest. Scale bars = 2 μm. d A schematic illustration of membrane proteins with different sizes of intracellular domains, including CD80-GFP, EGFP-GPI, TfR, Lyn-GFP, and EGFR-GFP, which were assessed for their lateral mobility on GCs. e Recovery kinetics of CD80-GFP, EGFP-GPI, TfR, Lyn-GFP and EGFR-GFP on GCs and control cells assessed by FRAP. For GCs with 4 to 20 wt% hydrogel densities, all examined membrane proteins had similar lateral mobility as on live cells. Error bars represent geometric mean with 95% confidence interval. f Membrane protein mobility on 40 wt% GCs relative to their corresponding mean mobility on live cells. Error bars represent geometric mean with 95% confidence interval (n = 7–12). Statistical analysis was performed using a two-tail Student t test, ****p<0.001
