Fig. 7
Expansion of antigen-specific T cells ex vivo and in vivo by G-DCs. a, b Antigen-specific CD8+ T-cell stimulation by G-DCs, live DCs, and glutaraldehyde-fixed DCs ex vivo. Error bars represent mean ± SEM. n = 3. c, d Antigen-specific CD8+ T-cell stimulation by G-DCs and live DCs in vivo. Error bars represent mean ± SEM. (n = 4 to 8). e, f G-DCs and non-gelated DCs were stored in PBS at 4 °C for 21 days (Day 21 G-DC and Day 21 DCs). In vivo antigen-specific CD8+ T-cell stimulation was examined with day 21 G-DC, day 21 DCs, and day 21 G-DC structurally disrupted by ultrasonication (homogenized G-DCs). T-cell expansion was monitored on day 6. CFSE-labeled T-cell groups (gray) are shown as negative controls. Error bars represent mean ± SEM, n = 3
