Fig. 6 | Nature Communications

Fig. 6

From: Etv6 activates vegfa expression through positive and negative transcriptional regulatory networks in Xenopus embryos

Fig. 6

Etv6 and Klf4 interact indirectly with each other. a In vivo immunoprecipitation assay showing the interaction between Etv6 and Klf4. Protein extracts from the somites of stage 22 embryos overexpressing etv6 mRNA were used for immunoprecipitation. Input lane: somite extracts from embryos injected with exogenous etv6 mRNA; IgG immunoprecipitation: negative control. b In vitro immunoprecipitation assay showing that Etv6 and Klf4 do not directly interact. The Etv6 and Klf4 proteins were synthetized from mRNA using the in vitro rabbit reticulocyte lysate translation system, extracted and used in immunoprecipitation assays with Etv6 antibodies (Etv6 IP) or Klf4 antibodies (Klf4 IP). c In vitro immunoprecipitation assay between two proteins known to physically interact with each other, Lmo2 and Ldb1, was performed as in (b) with Lmo2 antibodies (Lmo2 IP). The GFP protein serves as a negative control

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