Fig. 1 | Nature Communications

Fig. 1

From: Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development in mouse

Fig. 1The alternative text for this image may have been generated using AI.

Imbalanced Bmp/Nodal signalling and expansion of the PGC niche caused by Smad2 inactivation in the VE. a Representative images of p-Smad159 immunofluorescence (IF) staining of e5.5 embryos carrying the Blimp1-mVenus (BV) transgene, counter stained with DAPI. Dashed line indicates extent of proximal p-Smad159 staining in control embryos. The arrow indicates expanded p-Smad159 in the distal VE of Smad2ΔV embryos. b Whole-mount in situ hybridisation analysis of Gdf3 expression in control and Smad2ΔVE embryos at e5.5 and e6.0. c Nanog and Oct4 co-staining in e6.5 control and Smad2ΔVE embryos. d Brachyury IF in e6.5 control and Smad2ΔVE BV-expressing embryos. e Otx2 staining and BV expression at e6.5. All IF staining images were counter stained with DAPI. Scale bars = 100 μm

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