Fig. 3

Biofilm formation and hemolysis of the clinical ST378 isolates. a FEM pictures of the biofilm formed on the patient’s electrode. Scale bar left 5 µm and right 1 µm. b Quantitative in vitro biofilm assay of ST378 isolates. Isolates were grown in TSB 0.5% glucose in 96-well plates, and the washed biofilms were quantified by OD600 measurement. Statistical significance between the two groups was determined by Welch's t test (N = 60 t(46.40) = −10.513, P = 7.3 × 10-14). c Relative lysis of sheep blood erythrocytes by ST378 isolates indicated in percentages. The gray zone indicates the detection limit of 10% lysis below which the values were not included. Statistical significance between the two groups was determined by Welch's t test (N = 50 t(47.92) = 4.2857, P = 8.7 × 10-5). Group I and group II isolates are indicated in blue and red, respectively. Dark red indicates the clinical isolate retrieved from the blood culture. Averages with standard error of mean of at least three replicates are shown. ****P < 0.0001