Fig. 5
From: Dietary fatty acids fine-tune Piezo1 mechanical response
Dietary fatty acids alter Piezo1 channel gating in HMVEC. a Representative whole-cell patch-clamp recordings (at −60 mV) of control, MA, AA, EPA, and DHA (100 µM)-treated HMVEC elicited by mechanical stimulation. b Piezo1 current densities elicited by maximum displacement of control, MA, AA, EPA, and DHA (100 µM)-treated HMVEC. Bars are mean ± SD. Kruskal–Wallis and Dunn’s multiple comparisons test. c Boxplots show the mean, median, and the 75th to 25th percentiles of the displacement threshold required to elicit Piezo1 currents of control and MA, AA, EPA, and DHA (100 µM)-treated HMVEC. One-way ANOVA and Bonferroni test. d Ratio of the currents at the end of the displacement pulse to the peak current (ISTEADY/IPEAK) from macroscopic traces of control, MA, AA, EPA, and DHA (100 µM)-treated HMVEC. Bars are mean ± SD. One-way ANOVA and Bonferroni test. e EPA membrane content fold change of EPA-treated HMVEC supplemented with 100 µM for 18 h and 50 µM each day for 3 days, as determined by LC-MS. f Representative whole-cell patch-clamp recordings (at −60 mV) of EPA (50 µM each day for 3 days)-treated HMVEC elicited by mechanical stimulation; and ratio of the currents at the end of the displacement pulse to the peak current (ISTEADY/IPEAK) from macroscopic traces of control and EPA (50 µM each day for three days)-treated HMVEC. Bars are mean ± SD. Unpaired t-test. g MA, AA, and DHA membrane content fold change in MA, AA, and DHA (100 µM)-treated HMVEC as determined by LC-MS. Asterisks indicate values significantly different from control (∗∗∗p < 0.001 and ∗∗p < 0.01) and n.s. indicates not significantly different from the control. n is denoted above the x-axes
