Fig. 2
From: Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
Theozyme model construction and functional identification of the glycolaldehyde synthase. a The theozyme model interaction between glycolaldehyde and the active centers of different ThDP-dependent enzymes. The glutamate (glu) is tan; glycolaldehyde is cyan; ThDP is green. The distances between C2 atom in ThDP and glycolaldehyde carbon atoms are represented by d1 and d2. The green dot is magnesium ion. b The average distances (blue) between d1 and d2 in each protein are shown on the left. The amount of product (yellow) for the tested protein are shown on the right. The reaction was carried out by adding 1 mg mL−1 of the tested proteins and 2 g L−1 formaldehyde. ND no detection. Error bars represent s.d. (standard deviation), n = 3. c Protein expression of three functional candidates by using 1 mL of 1 OD cells. M: protein marker; 1, 3 and 5 represent protein expression without IPTG for 2UZ1, 3FZN, and 4K9Q, respectively; 2, 4, and 6 represent protein expression under IPTG inducing for 2UZ1, 3FZN, and 4K9Q, respectively; The red arrows point to the protein bands for 2UZ1, 3FZN, and 4K9Q, respectively. Source data are provided as a Source Data file.
