Fig. 7
From: Constructing a synthetic pathway for acetyl-coenzyme A from one-carbon through enzyme design
Assessing the SACA pathway by cell growth. a Cell growth assays using 0.4 g L−1 glycolaldehyde as supplemental carbon source. b Cell growth assays using 80 mg L−1 formaldehyde as supplemental carbon source. c Cell growth assays using 8 g L−1 methanol as supplementary carbon source. Cells initially were cultured in LB medium. IPTG was added to induce protein expression and then the supplemental carbon sources were added. OD600 was detected at different time points. SACA the strain contains the vector GALS-ACPS-PTA-28a, 28a the strain contains the empty vector of 28a, BsMDH-SACA the strain includes both BsMDH-pCDF and GALS-ACPS-PTA-28a vectors, BsMDH-28a the strain contains both BsMDH-pCDF vector and the empty vector 28a, No GALS the strain contains all genes in the pathway except GALS, + adding supplemental carbon source, − without supplemental carbon source, glycolaldehyde (GALD), formaldehyde (FALD), methanol (MeOH). Error bars represent s.d. (standard deviation), n = 3. Source data are provided as a Source Data file.
