Fig. 3
From: Cryo-EM structure of the human ferritin–transferrin receptor 1 complex
Characterization of human H-Ft wild type and mutants binding to CD71. a SPR sensograms of the interaction between the immobilized his-tagged CD71 receptor and H-Fts (wild-type and mutants), used as analytes. Fits are reported as black lines. Source data are provided as a Source Data file. b Ferritins uptake in HeLa cells has been quantified by flow cytometry. The percentage of cells internalizing AfFt-FITC (here used as negative control19), H-Ft-FITC, MutA-FITC, MutB-FITC, and MutC-FITC is shown as mean ± s.e.m. for n = 3 independent experiments. Source data are provided as a Source Data file. c Internalization of ferritins in HeLa cells observed at a ×60 confocal microscope, here shown as single FITC and overlay images with phase-contrast. Scale bars: 10 µm
