Table 2 Effect of mutation on biochemical properties of MB

From: Myoglobinopathy is an adult-onset autosomal dominant myopathy with characteristic sarcoplasmic inclusions

 

KD, O2 (µM)

k-H at pH 7.0 (h−1) a

k-H at pH 5.0 (h−1) b

E°’ by spectroelectr. (V vs SHE) c

E°’ by SWV (V vs SHE) c

kOX at pH 7.0 (min−1)

Heme SASA (nm2) d

WT

1.2 ± 1.1

0.22 ± 0.06

1.44 ± 0.09

+0.040 ± 0.005

−0.056 ± 0.015

1.96 ± 0.39

1.45 ± 0.04

His98Tyr

8.4 ± 2.5

1.19 ± 0.05

1.86 ± 0.05

+0.021 ± 0.005

−0.105 ± 0.015

1.97 ± 0.43

1.80 ± 0.05

  1. Conditions: a0.2 m phosphate buffer, pH 7.0, and 0.45 sucrose (37 °C); b0.2 m acetate, pH 5.0, and 0.45 m sucrose (37 °C); c0.2 m phosphate buffer, pH 7.0 (25 °C). dValues obtained from MD simulations
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