Fig. 2
From: Restoration of high-sensitivity and adapting vision with a cone opsin
Light response in isolated rd1 mouse retina with MW-Opsin in RGCs. a (Top) Average response to light flash of RGC population expressing MW-opsin (green) or rhodopsin (blue) in rd1 mouse retina. (Bottom) Raster plot of average response of rd1 mouse retina RGCs to 5 flashes of 100 ms duration light at 535 nm for MW-opsin (n = 59 cells) and 510 nm for rhodopsin (n = 27 cells) expressing in RGCs. b, c Light sensitivity for MW-opsin (N = 6 retinas) and rhodopsin (N = 4 retinas) in RGCs of rd1 mouse retina. Peak firing rate normalized to maximum at highest intensity. d, e Time-course of light response. Population average traces with time from light onset to max excitation (time to peak: 355 + 21 ms), exponential fits for excitatory phase (Tau ON: 112 + 25 ms) and decay (Tau OFF: 260 + 31 ms) and full width at half max (FWHM: 183 + 85 ms) (e) for MW-opsin (d; e, green; N = 5 retinas, nc = 104 channels), rhodopsin (e, blue; N = 7 retinas, nc = 134 channels) and wt (e, white; N = 3 retina, nc = 97 channels). f, g Dependence of MW-opsin light response on flash duration. f Representative retina light response (n = 117 cells): population average firing rate (top) and raster plot of unit responses (bottom). g Normalized peak responses for different stimulation durations (2 × 100 mW cm−2, N = 2 retinas, nc = 63 channels). Light intensity 2 × 10−1 mW cm−2 unless specified, Wavelength: λ = 535 nm (MW-opsin) or 510 nm (rhodopsin). N = number of retinas, nc = # of channels, n = number of cells/units. Cells are sorted units. Values are mean + SEM. Statistical significance assessed using Mann–Whitney U test (*p ≤ 0.05)
