Fig. 4
From: Restoration of high-sensitivity and adapting vision with a cone opsin
Light adaptation in RGC activity and visually guided behavior by MW-opsin. a–c MEA recordings in isolated retina of RGC light response mediated by MW-opsin in RGCs of rd1 mouse retina show sensitivity difference with retina adapted to dark versus light. a Light response decay (Tau OFF) as a function of flash intensity in dark versus light adapted condition (N = 3 retinas, nc = 88 channels). b Example intensity-response curve for representative retina first dark adapted (filled symbols) then light adapted (open symbols) (n = 56 cells). White light adaptation. ChR2 minimum value from Bi et al.13 and Sengupta et al.18. c Average (error bars are SEM) normalized Light response Index (LRI) at 3 flash intensities in same retina, first dark adapted and then light adapted (N = 3 retinas, nc = 88 channels). d–f Behavior shows light adaptation in visually guided tasks. d Schematic of adaptation to dark or light prior to testing of innate avoidance behavior or learned pattern discrimination behavior. e Proportion of time spent in the dark compartment (proportion of avoidance) under 100 μW cm−2 (bright light) or 1 μW cm−2 (dim indoor light) following 1 h. of adaptation to dark (N = 11 mice) or adaptation to light (white light; 1 mW cm−2/535 nm spectral component; ~50 μW cm−2; N = 11,13 mice). f Learned pattern discrimination of parallel bars spaced at distances of 1 versus 6 cm displayed at low (0.25 μW cm−2) or indoor (10 μW cm−2) light levels following 1 h. of adaptation to dark (N = 11 and 8 mice for each display) or light (white light; 1 mW cm−2/535 nm spectral component; 50 μW cm−2; N = 10 and 7 mice for each display). Performance was also reported in cohorts experiencing 4 and 8 h. of light adaptation (N = 7 mice). Dotted line denotes average performance of untreated rd1 control mice and performance (gray N = 5 mice) reproduced from Fig. 3h for reference and comparison. Wavelength: λ = 535 nm. N = # of animals, n = # of retina, nc = number of channels. Cells are identified as sorted units. Values are mean + SEM. Statistical significance assessed using Mann-Whitney U test (*p < 0.01). Student’s two-tailed t-test with Bonferroni correction: *p < 0.05
