Fig. 1

Transcription factor FgSR regulates ergosterol biosynthesis in F. graminearum. a Comparisons in colony morphology among the wild-type PH-1, ΔFgSR and the complemented transformant ΔFgSR-C grown on PDA, CM, or MM. b ΔFgSR displayed increased sensitivity to azole compounds but not to the compounds targeting the high osmolarity glycerol (HOG) pathway. A 5-mm mycelial plug of each strain was inoculated on PDA alone or supplemented with 5 μg ml^–1 tridiamenfon, 0.25 μg ml^–1 tebuconazole, 10 μg ml^–1 iprodione or 0.1 μg ml^–1 fludioxonil, and then incubated at 25 °C for 2 days. c Relative abundance of ergosterol in each strain after growth in YEPD for 36 h. Data presented are the mean ± s.d. (n = 3). Bars followed by the same letter are not significantly different according to a Fisher’s least significant difference (LSD) at P = 0.01. d The FgSR amino acid sequence shows a typical Zn(II)2-Cys6 zinc finger domain and a fungal specific transcription factor domain identified using the SMART protein database (http://smart.embl-heidelberg.de) and the NBCI protein database (https://blast.ncbi.nlm.nih.gov/Blast.cgi). e FgSR-GFP localized to the nucleus. Bar = 10 μm