Fig. 2

FgSR enriches at the promoters of three FgCYP51 genes and regulates their transcription in F. graminearum. a The transcription of FgCYP51A and FgCYP51B was regulated by FgSR, whereas, expression of FgCYP51C was modulated by FgSR only under the ergosterol-absent condition. Each strain was cultured in YEPD for 36 h, and then treated with 2.5 μg ml^–1 tebuconazole for 6 h (left panel). ΔFgErg4 was used to mimic the ergosterol-absent condition (right panel). The expression level of each FgCYP51 in PH-1 without treatment was referred to 1 and the FgACTIN gene was used as the internal control for normalization. Data presented are the mean ± s.d. (n = 3). Bars followed by the same letter are not significantly different according to a LSD test at P = 0.01. b FgSR binds to the promoters of three FgCYP51 genes, but the enrichment of FgSR at FgCYP51A and FgCYP51B is more than that at FgCYP51C. Each strain was incubated in YEPD liquid medium for 36 h. ChIP- and input-DNA samples were quantified by quantitative PCR assays with primers amplifying different regions indicated on the diagram of each FgCYP51 promoter. A control reaction was processed in parallel with rabbit IgG and PH-1 transformed only with GFP used as a negative control