Fig. 1
From: Calcium-activated 14-3-3 proteins as a molecular switch in salt stress tolerance
PKS5 interacts with SOS2 and phosphorylates SOS2Ser294. a Yeast two-hybrid assay showing that SOS2 interacts with PKS5. No interaction was observed when using an empty-vector control (BD-vector). b BiFC assay of the SOS2/PKS5 interaction in N. benthamiana. Plasmids containing YFPN-PKS5 and SOS2-YFPC or YFPN-AHA1 and SOS2-YFPC were co-transformed into N. benthamiana leaves. Two days post-infiltration, the YFP fluorescence was detected by confocal microscopy. Scale bars, 50 μm. c In vitro kinase assay showing that PKS5 phosphorylates SOS2Ser294. The recombinant proteins were incubated in kinase buffer at 30 °C for 30 min and separated by SDS–PAGE. Arrow indicates the target protein. d In vitro kinase assay showing that PKS5 phosphorylates SOS2-JD but not SOS2-JDS294A. JD, SOS2-JD; JDS294A, SOS2-JDS294A. CBB Coomassie brilliant blue, Autorad autoradiograph. Source data of c and d are provided in the Source Data file
