Fig. 5
RAD51/DMC1-assembly defects during in Swsap1 knockout (KO) mice. a Schematic of mouse Swsap1 genomic locus. The genomic locus of mouse Swsap1 shown to scale with KO construct (bottom). Exons are represented by boxes. b Swsap1 PCR genotyping. Exon1-specific forward, β-geo-specific forward and reverse primers were used. Wild-type and mutant genes show 3 and 0.75 kb fragments, respectively. c Swsap1 testis images. Representative images of Swsap1+/+ and Swsap1−/− testis are shown. Bar 100 µm. d Testis sections of wild-type and Swsap1 mutant mice. Cross sections of fixed testis were stained with HE. Top: Representative images of Swsap1+/+ and Swsap1−/− seminiferous tubules are shown. Bottom, quantification of atrophic tubules is shown. Ninety tubules in Swsap1+/+ and 101 tubules in Swsap1−/− were examined. e Cross sections of fixed ovary were stained with HE. Bar 500 µm. f RAD51 and SYCP3 immunofluorescence analysis of leptotene spermatocytes. Chromosome spreads were prepared and stained for RAD51 (green) and SYCP3 (red). Left, Representative images of Swsap1+/− and Swsap1−/− spermatocyte spreads are shown. Right, Quantification of RAD51 foci in leptotene spermatocytes. A number of RAD51 foci was counted per a nucleus. Data are mean ± s.d. Statistical significance was measured by Mann–Whitney’s U-test. Statistics and reproducibility; see accompanying Source Data. g DMC1 and SYCP3 immunofluorescence analysis of leptotene spermatocytes. Chromosome spreads were prepared and stained for DMC1 (green) and SYCP3 (red). Left, Representative images of Swsap1+/− and Swsap1−/− spermatocyte spreads are shown. Right, Quantification of DMC1 foci in leptotene spermatocytes. A number of DMC1 foci was counted per a nucleus. Data are mean ± s.d. Statistical significance was measured by Mann–Whitney’s U-test. Statistics and reproducibility; see accompanying Source Data
