Fig. 6
RAD51 assembly defects during mitosis in Swsap1 knockout (KO) mice. a, b Clonogenic survival assays of immortalized Swsap1+/+ and SWSAP1−/− cells exposed to different concentrations of camptothecin (CPT) and mitomycin C (MMC). A number of colonies with and without treatment of the DNA-damaging reagents were counted after 10–14 days culture. Data are mean ± s.e.m. Statistics and reproducibility, see accompanying Source data. c RAD51-positive cells in Swsap1+/+ and Swsap1−/− cells after CPT treatment. Swsap1+/+ and Swsap1−/− mouse embryonic fibroblast cells were treated with 100 nM CPT for 22 h. Cells were fixed and stained for RAD51 (green). Left, Representative images of RAD51-immunofluorescence staining. Bar 10 µm. Right, Quantification RAD51-positive cells in the indicated cell lines. More than 200 cells were counted. Data are mean ± s.d. Statistical significance was measured by Mann–Whitney’s U-test. Statistics and reproducibility; see accompanying Source data. d Quantification of γH2AX-positive cells in Swsap1+/+ and Swsap1−/− cells after CPT treatment. CPT-treated cells were stained for γH2AX. Data are mean ± s.d. Statistical significance was measured by Mann–Whitney’s U-test. Statistics and reproducibility; see accompanying Source Data
