Fig. 7
From: Rad52 prevents excessive replication fork reversal and protects from nascent strand degradation
Loss of RAD52 leads to persistence of unreplicated DNA. a Analysis of replication fork restart using DNA fibre assay. Cells were treated as indicated in the scheme on the top. The graph shown the percentage of each event. Representative images of DNA fibres fields are presented. Arrows denote stalled forks. (*P < 0.05; **P < 0.01; ***P < 0.001; ANOVA test; N = 140). b Analysis of the persistence of parental ssDNA (template gaps) during recovery from replication arrest. Cells were treated as indicated and analysed by the native iododeoxyuridine (IdU) assay. The graph shows the intensity of ssDNA staining in at least 100 nuclei from two independent experiments. Values are presented as means ± SE (****P < 0.0001; Mann–Whitney test; N = 141). Representative images are shown. c Analysis of RAD51 nuclear foci. Cells were treated with 2 mM hydroxyurea (HU), in the presence or not of the RAD52i, for 4 h and recovered as indicated, without or with RAD52i. The graph shows the percentage of nuclei with RAD51 foci. Data are presented as mean ± SE from three independent experiments. (ns, not significant; **P < 0.01; ANOVA test; N = 145). Representative images are shown. Scale bar represents 5 µm. Source data are provided as a Source Data file
