Table 1 Kinetic parameters for halogenations of selected substrates

From: Unusual substrate and halide versatility of phenolic halogenase PltM

Substrate

Halogen

Substrate/product

kcat (min−1)

Km (μM)

kcat/Km (min−1 μM−1)

3

Cl

3/Cl-3

2.3 ± 0.1a

(7.6 ± 1.3) × 10−2

30 ± 5

Cl-3/diCl-3

0.40 ± 0.01

0.11 ± 0.02

3.5 ± 0.6

Br

3/Br-3

1.9 ± 0.1

0.71 ± 0.2

2.7 ± 0.6

Br-3/diBr-3

0.38 ± 0.01

0.78 ± 0.17

0.49 ± 0.11

11

Cl

11/Cl-11

1.6 ± 0.1

8.4 ± 0.4

0.19 ± 0.01

Cl-11/diCl-11

0.18 ± 0.01

0.44 ± 0.02

0.41 ± 0.02

Br

11/Br-11

0.24 ± 0.02

(1.1 ± 0.2) × 103

(2.2 ± 0.2) × 10−4

16

Cl

16/Cl-16ab

0.35 ± 0.01

1.1 ± 0.3

0.31 ± 0.09

16/Cl-16b

0.22 ± 0.01

1.1 ± 0.3

0.19 ± 0.06

Cl-16a/diCl-16

0.95 ± 0.01

18 ± 5

(5.2 ± 1.4) × 10−2

Cl-16b/diCl-16

1.0 ± 0.1

15 ± 9

(7.0 ± 4.4) × 10−2

Br

16/Br-16a

0.47 ± 0.04

151 ± 20

(3.1 ± 0.5) × 10−3

16/Br-16b

2.2 ± 0.1

151 ± 20

(1.5 ± 0.2) × 10−2

Br-16a/diBr-16

0.21 ± 0.02

6.0 ± 1.2

(3.5 ± 0.8) × 10−2

Br-16b/diBr-16

2.0 ± 0.1

(1.3 ± 0.1) × 103

(1.5 ± 0.2) × 10−3

  1. aThe values of all mono-halogenation and dihalogenation rate constants kcat,1 and kcat,2, respectively, and Km for mono-halogenation and dihalogenation (defined as (kd,1 + kcat,1)/ka,1 and (kd,2 + kcat,2)/ka,2, respectively) were determined by nonlinear regression using DynaFit, as described in Methods
  2. bTwo distinct mono-halogenation products of the same reaction are denoted by labels a and b
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