Fig. 2

Determination of residues from HAd3K and EC2-EC3 involved in the interaction. a Surface of EC2-EC3 interacting with HAd3K. Aminoacids making contact with HAd3K are colored in light or dark green according to their interaction with monomer A or B, respectively. For a better view only two monomers of HAd3K are represented in cartoon. b Surface of HAd3K involved in receptor binding. HAd3K aminoacids involved in binding are highlighted in dark purple (EC2 contacts) and light purple (EC3 contacts). c EC2-EC3 primary sequence with aminoacids making contact with the knob are colored using same color code than in a. d Primary sequence of HAd3K with β-strand depicted with arrows. Contact-forming residues of HAd3K are depicted with same colors as in b. Asterisks (*) denote residues for which single mutation reduce DSG2 binding by more than 80% as decribed in Wang et al.²¹