Fig. 3 | Nature Communications

Fig. 3

From: CryoEM structure of adenovirus type 3 fibre with desmoglein 2 shows an unusual mode of receptor engagement

Fig. 3

A single D261A mutation in HAd3K is sufficient to abolish the receptor binding. a HAd3K incubation with an excess of EC2-EC3 produced in mammalian cell resulted in a complex eluted between 12 and 14 mL as shown by SEC and confirmed by SDS-PAGE. b Incubation of EC2-EC3 with the D261A mutated HAd3K resulted in a pic eluted latter (14–16 mL) corresponding to the two free components as shown by SDS-PAGE. Dotted rectangles on SDS-PAGE indicates the expected position of EC2-EC3 when in complex with the knob

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