Table 2 Detection of proteins involved in nitrite and NO metabolisms
ORF | Gene name | Annotation | Label-free quantification values | Log2 ratio | |||
|---|---|---|---|---|---|---|---|
| Â | Â | Â | Reactor I | Reactor II | Reactor III | II/I | III/I |
Nitrite transporter gene cluster | |||||||
 kuste3050 |  | Putative nitrite transporter | – | – | – | – | – |
 kuste3051 |  | Unknown protein | – | – | – | – | – |
 kuste3052 |  | Hypothetical protein | 1,158,000 | 336,465 | 1,202,850 | −1.78 | 1.84 |
 kuste3053 |  | Hypothetical protein | – | – | – | – | – |
 kuste3054 |  | Unknown protein | 335,650 | 196,738 | 267,740 | −0.77 | −0.33 |
 kuste3055 |  | Conserved hypothetical protein | 471,695 | 195,227 | 380,145 | −1.2727 | 0.9614 |
Nitrite reductase gene cluster | |||||||
 kuste4136 | nirS | Strongly similar to cd1 nitrite reductase NirS | 888,748 | 1,303,250 | 952,540 | 0.55 | −0.45 |
 kuste4137 | nirC | Hypothetical mono heme cytochrome NirC | – | – | – | – | – |
 kuste4138 | nirN | Conserved hypothetical NirN maturation protein | – | – | – | – | – |
 kuste4139 | nirJ | Similar to NirJ/MoaA/PpqE family of cofactor | – | – | – | – | – |
 kuste4140 | nirF | Similar to heme d1 biosynthesis protein NirF | – | – | – | – | – |
Hydroxylamine oxidoreductase-like and associated proteins | |||||||
 kustc0457 |  | Diheme cytochrome c protein | 1,341,000 | 2,005,250 | 904,210 | 0.58 | −1.15 |
 kustc0458 | hao | Similar to hydroxylamine oxidoreductase (hao) | 2,266,300 | 3,095,925 | 1,553,175 | 0.45 | −1.00 |
 kustc0694 | hdh | Hydrazine dehydrogenase | 10,175,950 | 12,669,000 | 6,790,225 | 0.32 | −0.90 |
 kustc1061 |  hox | Hydroxylamine:nitric oxide oxidoreductase | 6,259,425 | 9,912,625 | 4,616,800 | 0.66 | −1.10 |
Hydrazine synthase gene cluster | |||||||
 kuste2854 |  | Hypothetical (triheme) protein | 411,257 | 499,910 | 263,705 | 0.28 | −0.92 |
 kuste2855 |  | Hypothetical (hepta heme) protein | – | – | – | – | – |
 kuste2856 | fdoI | Similar to formate dehydrogenase, cytochrome b556 subunit | 216,773 | 93,117 | 238,043 | −1.22 | 1.35 |
 kuste2857 | hydG/atoC | Strongly similar to sigma 54 response regulator | – | – | – | – | – |
 kuste2858 |  | Unknown protein | 144,710 | 178,908 | – | 0.31 | – |
 kuste2859 | hzsC | Hydrazine synthase subunit C | 18,435,000 | 25,679,250 | 22,060,750 | 0.48 | −0.22 |
 kuste2860 | hzsB | Hydrazine synthase subunit B | 16,229,500 | 19,931,250 | 15,917,250 | 0.30 | −0.32 |
 kuste2861 | hzsA | Hydrazine synthase subunit A | 26,991,750 | 36,862,250 | 23,070,000 | 0.45 | −0.68 |
Nitrite:nitrate oxidoreductase gene cluster | |||||||
 kustd1699 |  | Hypothetical protein | 7,217,425 | 9,114,050 | 7,424,300 | 0.34 | −0.30 |
 kustd1700 | nxrA | Nitrite:nitrate oxidoreductase reductase subunit A | 11,748,750 | 18,509,500 | 11,701,750 | 0.66 | −0.66 |
 kustd1701 |  | Unknown protein | – | – | – | – | – |
 kustd1702 |  | Hypothetical protein | – | – | – | – | – |
 kustd1703 | nxrB | Nitrite:nitrate oxidoreductase reductase subunit B | 4,353,575 | 6,927,575 | 3,811,600 | 0.67 | −0.86 |
 kustd1704 |  | Unknown protein | 4,152,800 | 6,798,625 | 4,091,800 | 0.71 | −0.73 |
 kustd1705 | c556 | Conserved hypothetical (monoheme) protein | 1,131,375 | 1,773,775 | 733,265 | 0.65 | −1.27 |
 kustd1706 | uspA | Similar to universal stress protein UspA | – | – | – | – | – |
 kustd1707 |  | Conserved hypothetical (monoheme) protein | – | 81,870 | – | NaN | – |
 kustd1708 |  | Hypothetical (tetraheme) protein | 675,875 | 650,110 | 494,425 | −0.06 | −0.39 |
 kustd1709 |  | Hypothetical (diheme) protein | 426,010 | 287,390 | 481,298 | −0.57 | 0.74 |
 kustd1710 |  | Hypothetical (monoheme) protein | – | – | – | – | – |
 kustd1711 |  | Hypothetical (diheme) protein | – | – | 271,150 | – | – |
 kustd1712 | cydA | Similar to subunit 1 of alternative cytochrome bd quinol oxidase | – | – | – | – | – |
 kustd1713 |  | Conserved hypothetical protein | 4,680,375 | 5,748,750 | 6,014,050 | 0.30 | 0.07 |
