Fig. 2
From: Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition
Ectopic GATA4 expression induces lung cancer cell senescence. a Microphotograph of A549 cells infected lentivirus overexpressing GATA4. Red arrow-head highlighted the senescent cells. Scale = 100 μm. b β-galactosidase staining of A549 cells infected with GATA4 or EGFP expressing lentivirus. Representative pictures were shown. Scale = 100 μm. c, d Statistics of β-Galactosidase staining of A549 cells ectopically expressing GATA4 through lentivirus infection (c) or Doxycycline treatment of A549i cells (d) (n = 3 per group). e Western blot analysis of Doxycycline-inducible GATA4 expression in stable cell lines of H460, EKVX, and Hop62. f β-Galactosidase staining of indicated cell lines before and after GATA4 expression. g Statistics of f (n = 3 per group). h Western blot analysis of p15INK4b expression in GATA4-expressing A549 cells. i β-galactosidase staining of GATA4-expressing A549 knockdown with control or CDKN2B-targeting shRNAs (n = 3 per group). Bars are represented as mean ± SEM of the indicated number (n) of repeats. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t-test
