Fig. 5
From: Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition
GATA4 downregulates the TGFB2 mRNA level through multiple miRNAs. a Schematics of targeting sites on TGFB2 mRNA recognized by 14 miRNAs. b Relative expression level of miRNAs before and after GATA4 overexpression in A549i (through 2 μg/mL of Dox treatment) and A549 cells (through lentivirus infection) (n = 3 per group). c qPCR analysis of promoter fragment of designated gene from DNA samples prepared from control IgG or anti-FLAG (for pulldown GATA4) DNA samples. Quantity of GATA4 bond DNA was normalized by the value in control IgG-treated samples (n = 3 per group). d TGFB2 mRNA level in A549 cells overexpressing miR-32, miR-301b, miR-320A, or miR-590, respectively (n = 3 per group). e β-Galactosidase signal in GATA4 overexpressing A549 cells in the absence or presence of microRNA sponge (n = 3 per group). Bars are represented as mean ± SEM of the indicated number (n) of repeats. *P < 0.05, **P < 0.01, and ***P < 0.001 by Student’s t-test
