Fig. 3
From: Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision
Analysis of JellyOp light sensitivity and recovery in ESC-derived cardiomyocytes in vitro. a, b Relationship between normalized peak frequency increase and duration of light pulses (a) or applied photon-density (b) for light pulses with 30.9 (blue dots) and 0.309 (black dots) μW mm−2 (n = 6). c Representative frequency trace with long illumination (blue bar, 309 nW mm−2, 10 min, inactivation fit in red) and a subsequent second light pulse (20 s). d Percentage of recovery of frequency response after a short (20 s, black dots) or a long-lasting (10 min, blue dots) illumination (309 nW mm−2) analyzed with a second light pulse at various delay after the first light pulse. Error bars: S.E.M.
