Fig. 2

Probing the nucleation roles of CsgB in CsgA polymerization with CB-origami. a AFM height image of the formed oligomers (3.2 ± 0.3 nm in height) at the vertex of CA-origami and CB-origami, respectively (with 1.0 μM CsgA monomers). b Comparison of the nucleation efficiency between CB-origami (52.6 ± 5.3%) and CA-origami (14.3 ± 4.6%) by calculating the percentage of origami tethered with dot-like oligomer structures at the vertex (with 1.0 μM CsgA monomers); the data were collected by counting 100 origami structures for each group. All data points were averages of three independent experiments (n = 3) and presented as the mean ± standard error of the mean (s.e.m). **P < 0.01, two-tailed two-sample t-test. Source data are provided as a source data file. c, d AFM height and TEM images showing fibril (3.4 ± 0.4 nm in height) growing directly from the vertex of the CB-origami (with 2.0 μM CsgA monomers). The white arrows refer to the dot-like oligomers formed at the vertex. Scale bars: 100 nm. Note: AFM imaging was purposely carried out in tapping mode in liquid