Fig. 7 | Nature Communications

Fig. 7

From: Wnt1 silences chemokine genes in dendritic cells and induces adaptive immune resistance in lung adenocarcinoma

Fig. 7The alternative text for this image may have been generated using AI.

Wnt1 silencing attenuates Wnt pathway activation in human LUAD cDCs. a Human CD1−cDCs were sorted from primary lung adenocarcinomas and paired juxta-tumor healthy lung and analyzed by RNAseq. Gene Set Enrichment Analysis (GSEA) enrichment plot (n = 4 per group). b Primary human lung adenocarcinomas were dispersed and total cells cultured in the presence of Wnt1-silencing RNA (siWnt1) or control siRNA loaded nanoparticles (Up). Wnt1 silencing was confirmed by western blot (bottom). Representative histograms showing active b-catenin in cDCs (up) and extracellular CD107 in CD8+ T cells (bottom). Data are representative of 3 independent experiments. Source data are provided as a Source Data file. Sequencing data are available with the accession code GSE124199

Back to article page