Fig. 3
From: Primed histone demethylation regulates shoot regenerative competency
LDL3 activates genes for the acquisition of shoot traits upon shoot induction. Comparing transcriptome profiles of wild type (WT) before and after shoot induction (C14 vs C14S1 or C14 vs C14S7) identified genes that are up- or down-regulated (FC > 1.25 or < 0.8, p < 0.01) in response to shoot induction at early or late stages in WT (UGs_S1, DGs_S1, UGs_S7, and DGs_S7). a–d Expression changes of UGs_S1 (a), key developmental genes for SAM (b), DGs_S1 (c), and key developmental genes for RAM (d) upon shoot induction in WT and ldl3. Heat maps show relative expression levels for the genes by z-scores of read counts per million mapped reads (RPM). e, f Expression patterns of SAM reporters (green), pWUS::mTq2 (e), and pCLV3::dsRedN7 (f), in calli of WT and ldl3 before and after shoot induction. All panels are combined images of two sequential projections. Cellular outlines (magenta) were visualized with PI staining (e) or autofluorescence (f). Scale bars: 100 μm. See also Supplementary Fig. 3, Supplementary Data 3 and 4
