Fig. 3
SFX impairs sEV secretion through interfering with the ESCRT-dependent MVE biogenesis. a Heatmap of the selected transcriptome of SFX-treated MDA-MB231 cells compared to untreated control cells. Heatmap represents probe sets for transcripts expressed at significantly higher or lower levels after exposure to 100 μM SFX, respectively (FC = ±1.3 p value = 0.05). n = 3. b Advanced bubble chart shows enrichment of differentially expressed genes in the indicated signaling pathways. The x-axis label indicates gene ontology (GO) biological processes, and the y-axis label represents p value. c qRT-PCR analysis of various genes in 24 h SFX-treated MDA-MB231 (upper) and 6 h SFX-treated SK-MEL-28 human melanoma cells (lower). n = 3. d Immunoblot for the indicated proteins in MDA-MB231 cells. Beta-actin and Lamin B were used as loading controls for cell lysates and nuclear fraction, respectively. Right, Analysis of the relative protein intensity of proteins measured using a densitometer system. n = 3. Experiments were performed with 95% confluent cells. Significance was determined using an unpaired two-tailed Student’s t-test. ***p < 0.001, **p < 0.005 and *p < 0.05. Error bar, SD. Source data (c, d) are provided as a Source Data file
