Fig. 6
Behavior of dynamic microtubules (MTs) during end-conversion in vitro. a Schematics depicting different features of experimental kymographs. Fluorescent MT segments move away or toward the bead owing to the addition or loss of unlabeled tubulin from the bead-coupled MT plus-end, motor-dependent transport (gliding), and MT diffusion on bead surface. Vertical black line corresponds to a coverslip-immobilized bead (denoted by blue triangle above), which is often visible in the MT channel owing to the bead-attached motionless MTs. When such MTs were lacking, bead position was determined from green fluorescent protein (GFP) channel. The oblique black lines correspond to motions of the brightly labeled GMPCPP-stabilized MT seeds. Color bars on the right provide visual guides for interpretations of these motions. Arrowhead represents MT detachment; arrow represents end-to-wall transition event leading to MT diffusion. b Example kymographs for dynamic MT ends coupled to beads coated with indicated proteins together with CENP-E
