Fig. 8 | Nature Communications

Fig. 8

From: Microtubule end conversion mediated by motors and diffusing proteins with no intrinsic microtubule end-binding activity

Fig. 8

Kinesin-1 paired with Ndc80 in the microtubule (MT) wall-to-end transition assay. a Selected time-lapse images of a stabilized MT moving over a bead coated with Kinesin-1 and Ndc80 proteins in experiment carried out as in Fig. 2a. Numbers are time (s). Arrows show direction of MT gliding. Bar, 3 µm. b MT behavior as a function of the ratio of bead-bound proteins. Points are means ± SEM; curves are exponents (left) and hyperbolic functions illustrating the trends. Source data for (b, c) are provided as a Source Data file. c Average results for beads coated with Ndc80 and motors at ratios from 0.2 to 0.5. Bars show means ± SD for results from N independent trials, which are shown with gray dots. For CENP-E only, Ndc80 paired either with CENP-E, Kinesin-1, or Kinesin-1 at low adenosine triphosphate (ATP), N = 4, 3, 5, and 2, respectively. Numbers under each bar indicate the total number of observed events. Experiments were carried out in motility buffer supplemented with 2 mM ATP, except at low ATP concentration which was 20 µM

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