Fig. 2
PDATME dendritic cell (DC) exhibit a unique cytokine profile and promote IL-17 expression in naive CD4+ T cells. a–c Ova323–339 peptide-pulsed PDATME DC and splenic DC controls were co-cultured with Ova-restricted CD4+ T cells at a 1:5 ratio for 5 days. Representative (a) and quantitative (b) intracellular cytokine expression in the CD4+ T cells are shown. Data are representative of five independent experiments using 3–6 mice per arm, showing similar results. c Concentration of IL-17A in the supernatants of co-cultures were determined. Squares and triangles denote data points from individual mice, pooled from two independent experiments. d PDATME DC and splenic DC controls were plated at a density of 5 × 105 cells/ml without re-stimulation and supernatant cytokine concentrations were determined after 36 h in a cytometric bead array. Data are representative of experiments repeated >3 times. e Orthotopic pancreatic ductal adenocarcinoma (PDA) tumors and spleens were harvested from mice on day 25 post-implantation. Intracellular flow cytometric analysis of DC cytokine expression after 12 h of re-stimulation with LPS (1 μg/ml) is shown. Representative histograms are shown. Numbers represent median fluorescence indices (MFI). Data are representative of experiments repeated >3 times. f The frequency of IL-6, TGF-β1 and IL23p19, IL12/23p40 co-expression in PDATME DC and splenic DC controls were tested. Representative contour plots and quantitative data are shown (n = 5 per group). This experiment was repeated three times (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, t-test), SEM
