Fig. 6
From: FcγRIIb differentially regulates pre-immune and germinal center B cell tolerance in mouse and human
FcγRIIb expression controls bystander autoreactive GC B-cell expansion. a Serum dsDNA-specific IgG1 titer in KO-CD45.2, WT-CD45.2 or BTG-CD45.1 mice immunised for 8 days with SRBC. (R.U. = relative units) b RAG2KO and RAG2KO-mHEL recipients (both CD45.2) were irradiated and reconstituted with bone marrow from SWHEL-FcγRIIb KO-CD45.2, SWHEL-FcγRIIb WT-CD45.2 or SWHEL-FcγRIIb BTG-CD45.1 donor mice. Post reconstitution, chimeras were immunised with SRBC intraperitoneally and analysed 8 days later. c Number of HEL-specific follicular B cells in RAG2KO (left panel) and RAG2KO-mHEL (right panel) recipient mice. d Number of HEL-specific GC B cells in RAG2KO (left panel) and RAG2KO-mHEL (right panel) recipient mice. e Serum HEL-specific IgG1 titer in RAG2KO (left panel) and RAG2KO-mHEL (right panel) recipient mice. Two pooled experiments are shown. RAG2KO recipients: n = 5–8 mice per group; RAG2KO-mHEL recipients: n = 5–10 mice per group. The mean is represented and each dot corresponds to an individual mouse. The p-values were determined with the Kruskal–Wallis (KW) non-parametric test when comparing the three groups (KW: the p-value is indicated in purple for the RAG2KO-mHEL recipient. All KW tests were not significant for RAG2KO recipients with the exception of panel c for which KW p-value is indicated for the RAG2KO group as well) and with the Mann–Whitney non-parametric test when two conditions were compared (indicated by black stars). *p < 0.05; **p < 0.01. Comparisons generating non-significant p-values were indicated with “ns”. Source data are provided as a Source Data file
