Fig. 6
FadRSa exerts a repression on the transcription of the catabolic fatty acid metabolism/lipase gene cluster. a Reconstruction of the lipid degradation and fatty acid β-oxidation pathway with indication of the putative functions of genes in the Saci_1103-Saci_1126 gene cluster predicted to be involved in this metabolic pathway. This is based on genomic annotations. For each of these genes, differential expression in S. acidocaldarius MW001 ΔfadRSa, as measured by RNA-seq analysis, is shown in a heat map format expressed as the log2 of the fold change expression in the ΔfadRSa versus its isogenic wildtype strain. b Relative gene expression as determined by qRT-PCR for a subset of the genes of the Saci_1103-Saci_1126 gene cluster and for genes belonging to the sulfate reduction operon. Gray lines represent a fold change of 2 and 0.5, respectively. Error bars represent biological variation for triplicates (standard deviations). An asterisk indicates a p-value between 0.05 and 0.01, a double asterisk between 0.01 and 0.001, and a triple asterisk smaller than 0.001, as determined in a statistical t test. c Growth curves of MW001 and MW001 ΔfadRSa strains in Brock medium containing 2 mM hexanoate as sole carbon source. Values are averages of four biological replicates with error bars representing standard deviations. Doubling times (Tds) were calculated by modeling the exponential section of the growth curves. Representative curves are shown for multiple independently performed experiments
