Fig. 6
From: Reversible induction of mitophagy by an optogenetic bimodular system
Optogenetic-mediated mitophagy prevents apoptosis in a model of neurotoxicity. a ETNA (Embryonic Telencephalic NAïve) cells co-expressing Venus-iLID-ActA/AMBRA1-RFP-sspB were irradiated with pulsed (1 s light + 1 min dark) light for 4 h, then fixed and stained for nuclei (DAPI, blue) AMBRA1 (red) and Tom20 (cyan). Inset: ×4 magnification. Scale bar: 10 μm. b ETNA cells, transfected with Venus-iLID-ActA/AMBRA1-RFP-sspB or Venus-iLID-ActA/RFP-sspB (negative control), were irradiated with pulsed blue light (1 s light + 1 min dark) for 48 h or left in the dark upon treatment with 250 μM PQ. Cell lysates were prepared and HSP60 levels (mitochondrial marker) explored in three independent experiments. Actin was used as a loading control. The graph reports the means of the normalized densitometric ratio between HSP60 and actin. Data shown: mean ± S.E.M. Hypothesis test: ANOVA test. **p < 10−2. ***p < 10−3. Mr (kDa): Relative molecular mass expressed in kilodalton. c Venus-iLID-ActA/AMBRA1-RFP-sspB or Venus-iLID-ActA/RFP-sspB (negative control) ETNA cells were handled as described in (b). WB was performed to reveal PARP protein in cell lysates. Upper band: full length PARP. Lower band: cleaved PARP. Actin: loading control. The graph summarizes the densitometric quantification of cleaved/full PARP in three independent experiments. Data shown: mean ± S.E.M. Hypothesis test: ANOVA test. *p < 5 × 10−2. n.s. not statistically significant. Mr (KDa): Relative molecular mass expressed in kilodalton. Source data are provided as a Source Data file
