Fig. 6
From: Cortex-wide neural interfacing via transparent polymer skulls
Cortical microstimulation during wide-field Ca2+ imaging. a Top: Cartoon schematic of a microstimulation electrode inserted through a perforated See-Shell. Bottom: a wide-field image showing microstimulation electrode inserted at the primary motor cortex. Red circles indicate regions of interest (ROIs) analyzed. Scale bar indicates 2 mm. b Pseudocolor plots of normalized change in fluorescent intensity in response to primary motor cortex microstimulation. Top: under isoflurane anesthesia, bottom: awake. Post-stimulation time stamps are indicated at the bottom of each image. c Average normalized Ca2+ activity traces in response to microstimulation of primary motor cortex for different ROIs indicated in a. ROI 1: primary motor cortex and stimulation site; ROI 2: ipsilateral somatosensory cortex; ROI 3: ipsilateral visual cortex; ROI 4: contralateral motor cortex; ROI 5: contralateral somatosensory cortex; and ROI 6: contralateral visual cortex. Dashed lines indicate the time of stimulus. d Bar plots overlaid with the dot plots of full width at half maximum in the different ROIs under anesthetized and awake conditions. Error bars indicate s.d.; Student's t-test, *indicates p < 0.001
