Fig. 7
Misoprostol improves microcirculation and protects the heart in MI/R. WT mice were subjected to MI/R surgery. Vehicle, 50 or 100 μg/kg misoprostol was injected i.p. following LAD ligation before reperfusion, and additionally at 4, 8, 12 h of reperfusion. Representative photos of TTC stained Evans blue perfused hearts were shown (a). Infarct size (b) and AAR (c) were quantified. Ultrasonography was carried out at 24-h post MI/R (d). Ejection fraction (EF) and fractional shortening (FS) were quantified (e). Neutrophils (CD11b+Ly-6G+) in the ischemic heart tissue were detected by flow cytometry (f) and quantified as cell counts and percent of neutrophils in CD11b+ cells (g). Immunofluorescent staining of MPO was performed on the heart sections (h) and number of MPO-positive cells was quantified (i). Effect of misoprostol (10 μM) on leukocyte adhesion to ECs in vitro (j). In another set of mice, myocardial microcirculatory perfusion was determined during MI/R, and the representative perfusion images (k) and the statistical graph (l) were shown. One-way ANOVA with Dunn’s multiple comparison test (b, c, n = 6, 6, 7). Unpaired Student’s t test (e, n = 6, 7; g, n = 6, 8; i, n = 7; j, n = 9). Two-way ANOVA with Bonferroni’s test (l, n = 16, 15). Scale bar is 50 μm. Error bar indicates SEM
